Recombinant SARS-CoV-2 S-trimer Protein (Deltacron, BA.1×AY.4 recombinant, C-6His)

Specification

• Cat#: THP-0572
• Product Name: Recombinant SARS-CoV-2 S-trimer Protein (Deltacron, BA.1×AY.4 recombinant, C-6His)
• Description: Recombinant SARS-CoV-2 S-trimer Protein is generated using a Mammalian expression system. The target gene encoding Cys15-Gln1208 is expressed with a C-terminal 6His tag. It contains mutations T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, and L981F, identifying the Deltacron (BA.1×AY.4 recombinant) lineage from Europe. The protein is stabilized in the trimeric prefusion state via six proline substitutions and furin cleavage site modifications.
• Molecular Weight: 137.1kDa
• Introduction: The coronavirus spike (S) glycoprotein is essential for viral attachment to host cells at the onset of infection, particularly in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone mediates membrane fusion required for viral entry and cell fusion. It acts as a major immunogen and a target for entry inhibitors. SARS-CoV-2 reportedly infects human respiratory epithelial cells via the ACE2 receptor. The S protein is a large type I transmembrane protein with S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for cell recognition, while S2 contains fusion elements. The S protein is vital for inducing neutralizing antibodies, T-cell responses, and protective immunity.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g