Recombinant SARS-CoV-2 S-trimer Protein (CH.1.1, C-6His)

Specification

• Cat#: THP-0571
• Product Name: Recombinant SARS-CoV-2 S-trimer Protein (CH.1.1, C-6His)
• Description: Recombinant SARS-CoV-2 S-trimer Protein is produced by a Mammalian expression system. The target gene encoding Cys15-Gln1208 is expressed with a C-terminal 6His tag. It includes mutations T19I, LPP24-26del, A27S, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, G446S, L452R, N460K, S477N, T478K, E484A, F486S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, and N969K, characteristic of the SARS-CoV-2 CH.1.1 lineage (India origin). The protein features six proline substitutions and modified furin cleavage sites for trimer stabilization.
• Molecular Weight: 136.5kDa
• Introduction: The coronavirus spike (S) glycoprotein is essential for viral attachment to host cells at the onset of infection, particularly in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone mediates membrane fusion required for viral entry and cell fusion. It acts as a major immunogen and a target for entry inhibitors. SARS-CoV-2 reportedly infects human respiratory epithelial cells via the ACE2 receptor. The S protein is a large type I transmembrane protein with S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for cell recognition, while S2 contains fusion elements. The S protein is vital for inducing neutralizing antibodies, T-cell responses, and protective immunity.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g