Recombinant RSV Prefusion F-trimer Protein (strain A2)

Specification

• Cat#: THP-0541
• Product Name: Recombinant RSV Prefusion F-trimer Protein (strain A2)
• Description: Recombinant RSV (strain A2) Prefusion F-trimer Protein is produced by a Mammalian expression system. The target gene encoding Gln26-Leu513 is expressed with a C-terminal 6His tag. Mutations and furin cleavage site substitutions are introduced to stabilize the trimeric prefusion conformation and prevent cleavage.
• Molecular Weight: 56.1 kDa
• Introduction: Respiratory syncytial virus (RSV) is a significant respiratory pathogen. The Fusion (F) glycoprotein is a key vaccine antigen. It functions by refolding from a metastable prefusion state to a stable postfusion state, fusing viral and host membranes. The F0 precursor is typically cleaved by cellular proteases. Antibodies specific to the prefusion conformation are highly potent. An RSV F protein engineered to maintain a stable prefusion conformation and high expression levels represents a promising subunit vaccine candidate.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution containing 20mM His-HCl, 10% Trehalose, and 0.02% Tween 80, pH 5.5.
• Size: 50μg/500μg/1g