Recombinant HPIV-3 (strain Wash/47885/57) Prefusion F-trimer Protein

Specification

• Cat#: THP-0499
• Product Name: Recombinant HPIV-3 (strain Wash/47885/57) Prefusion F-trimer Protein
• Description: Recombinant HPIV-3 (strain Wash/47885/57) Fusion glycoprotein F0 is produced by a Mammalian expression system. The target gene encoding Gln19-Leu481 is expressed with a C-terminal 6His tag. Mutations are introduced to stabilize the trimeric prefusion state of the HPIV-3 Fusion glycoprotein.
• Molecular Weight: 55.8 KDa
• Introduction: Human parainfluenza virus type 3 (HPIV-3) is a major cause of lower respiratory tract infections in children. Viral entry and spread are initiated by the hemagglutinin-neuraminidase (HN) and fusion (F) envelope glycoproteins. HN binding to cellular receptors triggers conformational changes in F, leading to virus-cell fusion. The F protein is a type I integral membrane protein with an ectodomain, transmembrane anchor, and cytoplasmic tail. Synthesized as an inactive F0 precursor, it is cleaved into F1 and F2 subunits. The trimeric F structure exists on infectious virions in a metastable prefusion conformation with the hydrophobic fusion peptide buried internally.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution containing 20mM Hepes, 10% Trehalose, 150mM NaCl, and 0.05% Tween 80, pH 8.0.
• Size: 50μg/500μg/1g