Recombinant 2019-nCoV S-trimer Protein (B.1.617.2, C-6His)

Specification

• Cat#: THP-0473
• Product Name: Recombinant 2019-nCoV S-trimer Protein (B.1.617.2, C-6His)
• Description: Recombinant SARS-CoV-2 S-trimer Protein is expressed in a Mammalian system. The target gene encoding Cys15-Gln1208 is expressed with a C-terminal 6His tag. The protein includes mutations T19R, K77R, G142D, Δ156-157/R158G, A222V, L452R, T478K, D614G, P681R, D950N, RRAR to GSAS, K986P, and V987P, characteristic of the SARS-CoV-2 Delta variant (B.1.617.2 lineage) identified in India.
• Molecular Weight: 136.4kDa
• Introduction: The coronavirus spike (S) glycoprotein is critical for viral attachment to host cells at the beginning of the infection process, notably in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone can mediate membrane fusion necessary for viral entry and cell fusion. It serves as a major immunogen and target for entry inhibitors. Studies indicate that 2019-nCoV infects human respiratory epithelial cells by interacting with the human ACE2 receptor. The S protein is a large type I transmembrane protein composed of S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for recognizing cell surface receptors, while S2 contains elements required for membrane fusion. The S protein plays a key role in eliciting neutralizing antibodies, T-cell responses, and protective immunity. The Delta variant (B.1.617.2), bearing L452R and T478K substitutions in the RBD, is a highly prevalent strain detected globally.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution containing 20mM Tris-HCl, 8% Sucrose, and 0.05% Tween 80, pH 8.0.
• Size: 50μg/500μg/1g