Recombinant 2019-nCoV S-trimer Protein (501Y.V2, C-6His)

Specification

• Cat#: THP-0471
• Product Name: Recombinant 2019-nCoV S-trimer Protein (501Y.V2, C-6His)
• Description: Recombinant SARS-CoV-2 S-trimer Protein is generated using a Mammalian expression system. The target gene encoding Cys15-Gln1208 is expressed with a C-terminal 6His tag. It harbors the following mutations: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. These are characteristic of the SARS-CoV-2 Beta variant (20C/501Y.V2 or B.1.351 lineage).
• Molecular Weight: 136.2kDa
• Introduction: The coronavirus spike (S) glycoprotein is critical for viral attachment to host cells at the beginning of the infection process, notably in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone can mediate membrane fusion necessary for viral entry and cell fusion. It serves as a major immunogen and target for entry inhibitors. Studies indicate that 2019-nCoV infects human respiratory epithelial cells by interacting with the human ACE2 receptor. The S protein is a large type I transmembrane protein composed of S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for recognizing cell surface receptors, while S2 contains elements required for membrane fusion. The S protein plays a key role in eliciting neutralizing antibodies, T-cell responses, and protective immunity.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g