Recombinant 2019-nCoV S Protein RBD (E484Q, C-mFc)

Specification

• Cat#: THP-0439
• Product Name: Recombinant 2019-nCoV S Protein RBD (E484Q, C-mFc)
• Description: Recombinant SARS-CoV-2 S protein RBD Protein is generated using a Mammalian expression system. The target gene encoding Arg319-Phe541 with the E484Q mutation is expressed with a C-terminal mouse IgG1 Fc tag. The E484Q mutation identifies the SARS-CoV-2 B.1.617 lineage originally emerging in India.
• Molecular Weight: 51.5kDa
• Introduction: The coronavirus spike (S) glycoprotein is critical for viral attachment to host cells at the beginning of the infection process, notably in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone can mediate membrane fusion necessary for viral entry and cell fusion. It serves as a major immunogen and target for entry inhibitors. Studies indicate that 2019-nCoV infects human respiratory epithelial cells by interacting with the human ACE2 receptor. The S protein is a large type I transmembrane protein composed of S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for recognizing cell surface receptors, while S2 contains elements required for membrane fusion. The S protein plays a key role in eliciting neutralizing antibodies, T-cell responses, and protective immunity.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g