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Neutrophil Inhibitory Factor (NIF)

Cat# : THP-0240

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Catalog# Product Name Availability Size Price Qty
THP-0240 Neutrophil Inhibitory Factor (NIF) June 16, 2024 1 vial $3,998.00
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Cat#:  THP-0240
Product Name:  Neutrophil Inhibitory Factor (NIF)
Description:  Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host.
Species:  A. caninum
Molecular Weight:  Predicted Molecular Mass: 30 kDa SDS-PAGE: 55-65 kDa, reducing conditions
Source:  Mouse myeloma cell line
Introduction:  NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium. The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites. Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alpha M beta 2-mediated adhesion and degranulation. The NIF binding site within the alpha M beta 2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen. In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury.
Purity:  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Formulation:  Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT.
Biological Activity:  Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils. The ED50 for this effect is 0.1-0.4 μg/mL. Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA.
Reconstitution:  Reconstitute at 200 μg/mL in PBS.
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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  • Size: 100ug 500ug 1mg 5mg 10mg 100mg 500mg 1g
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