Biotinylated 2019-nCoV S Protein RBD-SD1 (C-Avi-6His)

Specification

• Cat#: THP-0405
• Product Name: Biotinylated 2019-nCoV S Protein RBD-SD1 (C-Avi-6His)
• Description: This Biotinylated SARS-CoV-2 S Protein RBD-SD1 is generated via a Mammalian expression system. The sequence encoding Arg319-Ser591 is expressed with Avi and 6His tags at the C-terminus.
• Molecular Weight: 33.2kDa
• Introduction: The coronavirus spike (S) glycoprotein is essential for viral attachment to host cells at the onset of infection, particularly in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone mediates membrane fusion required for viral entry and cell fusion. It acts as a major immunogen and a target for entry inhibitors. Studies indicate that 2019-nCoV infects human respiratory epithelial cells via the ACE2 receptor. The S protein is a large type I transmembrane protein consisting of S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for receptor recognition, whereas S2 facilitates membrane fusion. The S protein is key to inducing neutralizing antibodies, T-cell responses, and protective immunity.
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Stability: Bioactivity is maintained after 14 freeze-thaw cycles. Batch consistency is confirmed via ACE-2 (C-Fc)(C05Y) binding assays, showing no significant variation. Stability is verified after 14 days of storage at 4°C and 25°C.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g