Recombinant SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, N-6His)

Specification

• Cat#: THP-0544
• Product Name: Recombinant SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, N-6His)
• Description: Recombinant SARS-CoV-2 Nucleocapsid Protein is produced by an E. coli expression system. The target gene encoding Met1-Ala419 with mutations P13L, E31del, R32del, S33del, R203K, and G204R is expressed with an N-terminal 6His tag. These mutations identify the SARS-CoV-2 Omicron variant (B.1.1.529 lineage) from South Africa.
• Introduction: The Coronavirus Nucleocapsid (N) protein is essential for viral RNA synthesis and possesses RNA chaperone activity facilitating template switching. As the most abundant viral protein, N protein packages the positive-strand viral genome into a helical ribonucleocapsid (RNP). It plays a fundamental role in virion assembly via interactions with the viral genome and membrane protein M. Additionally, it enhances the efficiency of subgenomic viral RNA transcription and replication. Due to its high sequence conservation and strong immunogenicity, N protein is a preferred diagnostic target.
• Purity: Purity exceeds 95% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution containing 20mM Tris-HCl, 300mM NaCl, and 1mM EDTA, pH 8.0.