Tumor Necrosis Factor alpha (TNF-alpha)

Specification

• Cat#: THP-0220
• Product Name: Tumor Necrosis Factor alpha (TNF-alpha)
• Description: Recombinant TNF-alpha protein (Val 77-Leu 233) is expressed in E.coli, with an initial Met at the N-terminus.
• Species: Human
• Molecular Weight: The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
• Source: E. coli
• Introduction: Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha.
• Purity: >95 % as determined by SDS-PAGE
• Endotoxin: <5EU per 1 mg of the peptide by the LAL method
• Biological Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL. Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated bound to Humira was 0.1 nM.
• Application: TNF-alpha is involved in fighting against the tumorigenesis.