Recombinant Human IGF1 and IGFBP3 Protein

Specification

• Cat#: THP-0351
• Product Name: Recombinant Human IGF1 and IGFBP3 Protein
• Description: Mecasermin rinfabate is an aqueous solution containing a binary protein complex of human insulin-like growth factor-1 (rhIGF-1) and human insulin-like growth factor-binding protein-3 (rhIGFBP-3), both produced by recombinant DNA technology. rhIGF-1 and rhIGFBP-3 are produced by two separate E. coli strains: one containing the human gene for insulin-like growth factor-1 (IGF-1), the other containing the human gene for insulin-like growth factor-binding protein-3 (IGFBP-3). IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges and a molecular weight of 7649 daltons. The amino acid sequence of the rhIGF-1 protein is identical to that of endogenous human IGF-1. IGFBP-3 consists of 264 amino acid residues with a molecular weight of 28,732 daltons. The amino acid sequence of the rhIGFBP-3 protein is identical to that of endogenous human IGFBP-3. Endogenous IGFBP-3 contains 18 cysteine residues that are all paired in disulfide bonds to form the biologically active molecule, but the pairings have not been fully elucidated. IGFBP-3 from human plasma is glycosylated, whereas rhIGFBP-3 produced in E. coli is non-glycosylated. Glycosylated and nonglycosylated IGFBP-3 bind IGF-1 with similar affinities.
• Species: Human
• Molecular Weight: The rhIGF-1 and rhIGFBP-3 proteins are complexed in a 1:1 molar ratio for formation of mecasermin rinfabate with a molecular weight of 36,381 daltons.
• Source: E. coli
• Purity: >99% by SDS-PAGE
• Endotoxin: <0.001EU/ug by the LAL method.
• Storage: Mecasermin rinfabate is temperature sensitive and should remain frozen at -15.5℃ to -1.5℃ until time of use. It can be stored in a freezer for up to 3 months.
• Concentration: 60mg/ml