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P53 Tumor Suppressor

Cat# : THP-0259

Product Datasheets COA :
Catalog# Product Name Availability Size Price Qty
THP-0259 P53 Tumor Suppressor March 01, 2024 1 vial $3,998.00
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Cat#:  THP-0259
Product Name:  P53 Tumor Suppressor
Description:  P53 Tumor Suppressor is E. coli-derived human p53 protein.
Species:  Human
Molecular Weight:  44 kDa
Source:  E. coli
Introduction:  p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range of factors that promote DNA repair mechanisms or apoptosis, including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase that catalyzes p53 ubiquitination and proteasome-mediated degradation.
Purity:  >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Formulation:  X mg/ml (X μM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% Glycerol (v/v), 30 µM ZnCl2.
Biological Activity:  Recombinant Human p53 is ideal for use as a control substrate for in vitro Ubiquitin conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human p53 concentration of 0.5-2.5 μM.
For research use only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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