Interleukin-12 (IL-12)

Specification

• Cat#: THP-0230
• Product Name: Interleukin-12 (IL-12)
• Description: Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40 kDa protein containing 306 amino acid residues.
• Species: Human
• Molecular Weight: 40 kDa
• Source: CHO cells
• Introduction: As an influential heterodimeric pleiotropic cytokine, IL-12 is key in regulating certain immune responses. This is done through its regulation of adaptive type-1 cell-mediated immunity: a pathway that aids in protection against many viral agents. It is primarily produced through the stimulation of white blood cells in human tissue known as macrophages, monocytes or dendritic cells and occurs naturally in the body, as a response to factors such as invading pathogens, cellular damage interacting with activated T-cells. Having a large impact over both T-cells and natural killer cells (or NK cells), IL-12 has been identified as having strongly positive applications for fighting cancer.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Biological Activity: Human IL-12 p40 has no IL-12 (p70) antagonist activity.
• Reconstitution: We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration . This solution can then be diluted into other aqueous buffers and stored at 4 centigrade for 3 months or below -10 centigrade for future use.
• Application: Having a large impact over both T-cells and natural killer cells (or NK cells), IL-12 has been identified as having strongly positive applications for fighting cancer.