Diphtheria Toxin Mutant (CRM197)

Specification

• Cat#: THP-0239
• Product Name: Diphtheria Toxin Mutant (CRM197)
• Description: CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine.
• Species: Corynebacterium diphtheriae
• Molecular Weight: 58.4 kDa
• Source: Pseudomonas fluorescens
• Introduction: CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. Pfenex CRM197 is a recombinant form expressed in Pseudomonas fluorescens using the Pfenex Expression TechnologyTM platform.
• Purity: >95% CRM197 by SDS-PAGE
• Formulation: CRM197 is lyophilized from a 0.2 micro-filtered solution containing 4 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80.
• Dimers: < 5%

Pharmaceutical Information

• Applications: It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections.
• Examples of Clinical Use: Carrier protein in a number of approved conjugate vaccines
• Affected organisms: Humans