Betacellulin (BTC)

Specification

• Cat#: THP-0274
• Product Name: Betacellulin (BTC)
• Description: Recombinant Human Betacellulin (BTC) Protein (Asp32-Tyr111) is produced by E. coli expression system. N-terminal Sequence Analysis: Asp32.
• Species: Human
• Molecular Weight: Predicted Molecular Mass: 9 kDa; SDS-PAGE: 15 kDa, reducing conditions.
• Source: E. coli
• Introduction: The Betacellulin protein is encoded by the BTC gene on chromosome 4 at locus 4q13-q21 in humans. It is a member of the epidermal growth factor (EGF) protein family. It promotes the secretion of insulin and pancreatic cell proliferation, plus retinal vascular permeability. There is a possible association between type 2 diabetes and mutations of the gene. Another disease associated with BTC is squamous cell carcinoma. BTC was first found in mice but was subsequently discovered in humans too. Betacellulin is used in stem cell research because it induces neural stem cell proliferation. It is a mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin has been tested as part of gene therapy with neurogenin 3 to measure its effect on metabolic problems in diabetic mice and could help to reverse major issues. It could help to reduce gluten intolerance. Plus, it has been shown to promote retinal vascular permeability, which is also useful for diabetes treatment as type 2 diabetes can cause vision loss due to diabetic maculopathy. Mouse models have shown that BTC can result in accentuated retinal vascular permeability in mice with diabetes. There have also been studies showing that BTC regulates hair follicle development and regulates epidermal homeostasis too.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.6 ng/mL.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Application: Betacellulin could help to reduce gluten intolerance. Plus, it has been shown to promote retinal vascular permeability, which is also useful for diabetes treatment as type 2 diabetes can cause vision loss due to diabetic maculopathy.