PEDF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5 kDa. The Human PEDF is purified by proprietary chromatographic techniques.
PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. PEDF is a 50,000 dalton glycoprotein created and secreted in many tissues all the way through the body. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, PEDF is capable to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuro-protective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers.
>95% as determined by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder. The sterile filtered concentrated (1mg/ml) protein solution was lyophilized with 20mM sodium phosphate buffer & 150mM NaCl pH-7.4.
It is recommended to reconstitute the lyophilized PEDF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. The neuro-protective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins.
Examples of Clinical Use:
Having neurotrophic, anti-angiogenic, and anti-tumorigenic properties.
PEDF, also known as Pigment pigment derived factor, is an embryonic development regulatory factor secreted by retinal pigment epithelial cells. In the initial research, people found that PEDF is a neurotrophin with inhibitory effect in tumor cells.
Functions of PEDF
PEDF has multiple roles in physiological and pathophysiological processes. The main function of PEDF is to regulate neuronutrition, cell differentiation, and cell proliferation. PEDF is also involved in neuroprotection, inflammation, and immune regulation processes. PEDF is crucial for the development, function, and survival of retina and brain tissue. In addition, PEDF is also involved in the occurrence and development of many diseases, such as diabetes, cardiovascular diseases, tumors, neurodegenerative diseases, etc.
PEDF related signaling pathways
PEDF primarily exerts its biological functions by activating downstream signaling pathways. Research has shown that PEDF mainly functions through pathways such as PI3K/Akt, ERK1/2, JNK, p38-MAPK, and Smad signaling pathways in the processes of neurotrophic, cell differentiation, and cell proliferation. PEDF also passes through TGF- β Signal pathways and NF- κ The B pathway is involved in cardiovascular disease and inflammatory processes.
PEDF related diseases
Diabetes: PEDF reduces insulin secretion by inhibiting the flow of intracellular calcium ions, leading to insulin resistance and diabetes. In addition, PEDF also plays an important role in the occurrence of diabetes complications, such as diabetes retinopathy and diabetes nephropathy.
Cardiovascular disease: PEDF inhibits the proliferation and migration of vascular endothelial cells and smooth muscle cells, thereby hindering angiogenesis and vascular restenosis. PEDF also has a protective effect on the myocardium during myocardial ischemia-reperfusion.
Tumor: PEDF has a complex mechanism of action in the occurrence and development of tumors. PEDF inhibits tumor growth by inhibiting the proliferation and invasiveness of tumor cells, and promoting tumor cell apoptosis. However, PEDF can also promote tumor growth and proliferation by promoting new angiogenesis, catalyzing the migration and heterogeneity of tumor cells, and other means.
The Application of PEDF in Medicine
Treatment of diabetes and diabetes complications PEDF inhibits the flow of calcium ions and reduces insulin secretion, which can be used to control diabetes and diabetes complications.
Inhibiting the growth and invasiveness of tumor cells: PEDF has an inhibitory effect on the proliferation and invasiveness of tumor cells. Therefore, PEDF can be used to treat various tumors.
PEDF plays an important role in promoting neuronutrition and protecting the nervous system in the processes of neuroprotection, inflammation, and immune regulation. Therefore, PEDF can be used to treat neurological diseases.
List of PEDF related investigational drugs
1. Dipeptide MIMETIK-MV-1 is a modified PEDF molecule that can inhibit the proliferation and metastasis of cancer cells. The drug has achieved certain success in the treatment of liver cancer and breast cancer.
2. Ionic tripeptide GK-1 is an expression product of PEDF gene and is considered to have the potential to treat ophthalmic diseases. The drug can promote the survival of retinal ganglion cell and prevent the occurrence of retinopathy.
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