Interleukin-10 (IL-10)

Specification

• Cat#: THP-0233
• Product Name: Interleukin-10 (IL-10)
• Description: Recombinant human IL-10 is a non-glycosylated protein, containing 161 amino acids and having a molecular mass of 16.6 kDa.
• Sequences: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
• Species: Human
• Molecular Weight: 16.6 kDa
• Source: E. Coli
• Introduction: Interleukin 10 (IL-10) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-γ, TNF-α, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells.
• Biological Activity: The activity is determined by the dose-dependant proliferation of mouse MC-9 cells and is typically less than 0.5 ng/mL.
• Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Application: IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-γ, TNF-α, IL-2, and IL-3, IL-4, and GM-CSF.