Brain-Derived Neurotrophic Factor (BDNF)

Specification

• Cat#: THP-0238
• Product Name: Brain-Derived Neurotrophic Factor (BDNF)
• Description: Brain-derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids (and an N-terminal Met) and having a total molecular mass of 27kDa.
• Sequences: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
• Species: Human
• Molecular Weight: 27 kDa
• Source: E. Coli
• Introduction: BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
• Purity: >97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2µg/ml.
• Reconstitution: It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Application: BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns.