Transforming Growth Factor-Alpha (TGFA)

Specification

• Cat#: THP-0242
• Product Name: Transforming Growth Factor-Alpha (TGFA)
• Description: TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa. The TGFA is purified by proprietary chromatographic techniques.
• Sequences: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
• Species: Human
• Molecular Weight: 6 kDa
• Source: E. Coli
• Introduction: Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.
• Purity: >97.0% as determined by SDS-PAGE.
• Biological Activity: The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.
• Reconstitution: It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Application: Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells.