Recombinant Trypanosoma cruzi 1F8 Chagas Antigen, His-tagged

Specification

• Cat#: FCS-027
• Product Name: Recombinant Trypanosoma cruzi 1F8 Chagas Antigen, His-tagged
• Description: Recombinant Chagas 1F8 protein (Epitope of the flagellar calcium-binding protein FCABP of Trypanosoma cruzi) produced in E. coli, is a polypeptide chain having a total of 234 a.a (includes his tag). The protein is fused to a 6 × His tag having a total Mw of 25.8 kDa and a theoretical pI of 5.39.
• Species: Trypanosoma cruzi
• Molecular Weight: 25.8 kDa
• Source: Escherichia Coli.
• Introduction: Flagellar calcium-binding protein (Protein FcaBP) is a protein which may be involved in swift motility of Trypanosoma, having an imperative role in flagellar structure and calcium metabolism, as it exhibits 4 EF-hand type (helix-loop-helix) structural domains, of which 3 may contain calcium-binding sites. Calcium has a vital role in the motility of Trypanosoma. The subcellular localization of Protein FcaBP is in the flagella of T. cruzi.
• Purity: Protein is >90% pure as determined by SDS PAGE.
• Formulation: 1F8 Chagas antigen was lyophilized from 20mM Hepes, 300mM NaCl, pH-8, and varying 284mM imidazole.
• Stability: Store lyophilized 1F8 Chagas antigen at 2-8°C.After reconstitution, store at -20°C.Prevent freeze/thaw cycles.

Pharmaceutical Information

• Applications: Reacts with sera from infected patients, Useful for immunoassays.