Recombinant HCV Genotype 1a NS5 Antigen, GST-tagged

Specification

• Cat#: HCV-213
• Product Name: Recombinant HCV Genotype 1a NS5 Antigen, GST-tagged
• Description: The E. coli derived recombinant protein contains the HCV NS5 Genotype 1a immunodominant regions, amino acids 2322-2423 having a total Mw of 38.34 kD which includes a 26 kDa GST tag.
• Species: HCV
• Molecular Weight: 38.34k
• Introduction: HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).
• Purity: HCV NS5 Genotype-1a protein is >95% pure as determined by 10% PAGE (coomassie staining).
• Formulation: 50mM Tris, pH 8 & 5mM EDTA.
• Stability: HCV NS5 Genotype-1a although stable at 4°C for 1 week, should be stored below -18°C.Please prevent freeze thaw cycles.

Pharmaceutical Information

• Applications: HCV NS5 Genotype-1a antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.