Recombinant HBV Pre-S Antigen, His-tagged

Specification

• Cat#: H3N2-308
• Product Name: Recombinant HBV Pre-S Antigen, His-tagged
• Description: Recombinant Hepatitis B Virus Pre-S produced in E. coli is a full length Pre-S of HBV subtype adw2, containing 174 amino acids, having a molecular weight of about 25 kDa including a vector sequence and linked with a 6 × His tag at C-terminus.
• Species: HBV
• Molecular Weight: 25 kDa
• Source: Escherichia Coli.
• Introduction: Hepatitis B virus surface gene encodes 3 proteins: small (HBs), middle (MHBs) and large (LHBs) surface protein. These 3 proteins are translated from distinct start codons, however share a common reading frame and stop codon. HBs contains 226 amino acids, it is a major viral envelope component. MHBs has extra 55 amino acids (Pre-S2) located to the N-terminus of HBs, LHBs has an additional 119 amino acids (Pre-S1). Pre-S is a region is comprised of Pre-S1 and S2, Pre-S1 has a vital role in receptor recognition, while Pre-S2 plays a part in translocation of virus into host cell. Clinically, HBV pre-S is linked with HBsAg and HBV DNA copies, which indicates active replication of virus, reduction of pre-S is typically associated with decreased HBV DNA copies, pointing to an improved predictor of treatment. In addition, Pre-S is a potential vaccine under development to enhance currently used HBsAg vaccine.
• Purity: Protein is >95% pure as determined by 12% PAGE (coomassie staining).
• Formulation: Sterile Filtered solution containing PBS, 1M urea and 0.05% sodium azide.
• Stability: HBV Pre-S although stable at 4°C for 1 week, should be stored below -18°C.Please prevent freeze thaw cycles.
• Physical Appearance: Sterile Filtered solution.
• Usage: All the products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Pharmaceutical Information

• Applications: Immunoassay.