Recombinant HBV core Antigen, His-tagged

Specification

• Cat#: VAC-0207
• Product Name: Recombinant HBV core Antigen, His-tagged
• Description: The E. coli derived recombinant protein contains the HBV core immunodominant region amino acids 1-186, and fused to a His tag at N-terminus.
• Species: HBV
• Introduction: Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
• Purity: HBV Core protein is >90% pure as determined by 10% PAGE (Coomassie staining).
• Formulation: 25mM Tris-HCl pH-8.0, 1.5mM EDTA, 1.5mM Urea and 50% glycerol.
• Stability: HBV Core protein although stable at 4°C for 1 week, should be stored below -18°C.Please prevent freeze thaw cycles.
• Usage: All the products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.