Recombinant CMV Pp65 Antigen

Specification

• Cat#: CMV-036
• Product Name: Recombinant CMV Pp65 Antigen
• Description: The E. coli derived 62.8 kDa recombinant protein contains the CMV Pp65 (UL83) immunodominant regions, having 561 amino acids.
• Species: CMV
• Molecular Weight: 62.8 kDa
• Source: Escherichia Coli.
• Introduction: CMV is part of the Betaherpesvirinae subfamily of Herpesviridae; including herpes simplex virustypes 1 and 2, varicella-zoster virus, and Epstein-Barrvirus. The herpesviruses has the common ability to stay latentover long periods of time. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.
• Purity: CMV Pp65 protein is >95% pure as determined by SDS-PAGE.
• Formula: 25mM Tris-Hcl pH 8, 8M Urea, 5mM bMe.
• Stability: CMV Pp65 protein although stable at 4°C for 1 week, should be stored below -18°C.Please prevent freeze thaw cycles.
• Application: CMV Pp65 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.