Recombinant CHIKV E1 Antigen

Specification

• Cat#: HCV-175
• Product Name: Recombinant CHIKV E1 Antigen
• Description: Recombinant Chikungunya E1 produced in E. coli having a molecular weight of 48 kDa.
• Species: CHIKV
• Molecular Weight: 48 kDa
• Source: Escherichia Coli.
• Introduction: Chikungunya is an infection caused by the chikungunya virus which is passed to humans by two species of mosquito of the genus Aedes: A. albopictus and A. aegypti. Animal reservoirs of the virus include monkeys, birds, cattle, and rodents. The features of the disease are a sudden onset of fever 2-4 days after exposure. The fever typically lasts 2-7 days, while the associated joint pains usually last weeks or months but sometimes years. The mortality rate is a little less than 1 in 1,000.The disease has occurred in outbreaks in Asia, Europe and the Americas since 2004. CHIKV is a single-stranded positive-sense RNA genome, 11,800 nts long which encodes 2 open reading frames. The nucleocapsid is tightly enveloped by a host-derived lipid bilayer (envelope) supporting the virus-encoded envelope proteins. 80 glycoprotein spikes are C- terminally anchored within the viral envelope. The structural polyprotein is translated from a viral sub genomic mRNA, while as the 5 structural proteins (capsid, E3, E2, 6K, E1) are translated as a single polyprotein, from which capsid (C) is cleaved off to encapsidate. The envelope polyprotein precursor E3-E2-6K-E1 is translocated to the endoplasmatic reticulum. Polyprotein is processed by host signalases, resulting in E3, E2 & E1 forming viral hetero-trimeric spikes. The viral spikes majorly containsE2 and E1 facilitate cell receptor recognition, cell entry thru pH-dependent endocytosis and support viral budding.
• Purity: Protein is >90% pure as determined by SDS-PAGE.
• Formulation: Sterile Filtered solution containing PBS.
• Stability: CHIKV E1 although stable at 4°C for 1 week, should be stored below -18°C.Please prevent freeze thaw cycles.
• Physical Appearance: Sterile filtered colorless solution.
• Usage: All the products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.