Recombinant 2019-nCoV S Protein HR1 (N-6His-Sumo Tag)

Specification

• Cat#: THP-0416
• Product Name: Recombinant 2019-nCoV S Protein HR1 (N-6His-Sumo Tag)
• Description: Recombinant SARS-CoV-2 S protein HR1 Protein is produced in an E. coli expression system. The target gene encoding Gly910-Glu988 is expressed with N-terminal 6His and Sumo tags.
• Molecular Weight: 20.8kDa
• Introduction: The coronavirus spike (S) glycoprotein is essential for viral attachment to host cells at the onset of infection, particularly in severe acute respiratory syndrome (SARS). The SARS-CoV S glycoprotein alone mediates membrane fusion required for viral entry and cell fusion. It acts as a major immunogen and a target for entry inhibitors. SARS-CoV-2 reportedly infects human respiratory epithelial cells via the ACE2 receptor. The S protein is a large type I transmembrane protein with S1 and S2 subunits. S1 contains the receptor-binding domain (RBD) for cell recognition, while S2 contains fusion elements. Following RBD binding to ACE2, the heptad repeat 1 (HR1) and 2 (HR2) domains in the S2 subunit interact to form a six-helix bundle (6-HB) fusion core, bringing viral and cellular membranes into close proximity for fusion. The S protein is vital for inducing neutralizing antibodies, T-cell responses, and protective immunity.
• Purity: Purity exceeds 90% as assessed by reducing SDS-PAGE (QC verified).
• Storage: Upon reconstitution, store samples at -20°C or lower. The product remains stable for a minimum of 24 months under recommended storage conditions.
• Shipping Conditions: Transported using dry ice packs. Immediate storage at the specified temperature is required upon receipt.
• Formulation: Provided as a 0.2 μm filtered solution in PBS, pH 7.4.
• Size: 50μg/500μg/1g