Leukocyte Elastase Inhibitor (LEI)

Specification

• Cat#: THP-0265
• Product Name: Leukocyte Elastase Inhibitor (LEI)
• Description: Recombinant Leukocyte Elastase Inhibitor (LEI) Protein(His17-Glu332) is produced by E. coli expression system and fused with two N-terminal Tags, His-tag and T7-tag.
• Sequences: MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEFELRR QAC- HTLS ESSPTGNIFF SPFSISSALA MVFLGTKGTT AAQLSKTFHF DSVEDVHSRF QSLNAEVSKR GASHTLKLAN RLYGEKTYNF LPEFLTSTQK MYGADLAPVD FQHASEDARK EINQWVKGQT EGKIPELLAV GVVDSMTKLV LVNAIYFKGM WEEKFMKQDT TDAPFRLNKK NTKSVKMMYQ KKKFFFGYIS DLKCKVLEMP YQGGELSMVI LLPEDIEDES TGLKKIEEQI TLEKLREWTK RENLENIDVH VKLPRFKIEE SYILNSNLGR LGLQDLFNSS KADLSGMSGS RDLFISKIVH KAFVEVNEEG TE
• Species: Rat
• Molecular Weight: 42 kDa
• Source: E. coli
• Introduction: Leukocyte Elastase Inhibitor (LEI) is a member of the clade B of SERPINS. It is an intracellular protein and acts primarily to protect the cell from proteases released into the cytoplasm during stress. Its role in inflammation is clear due to its involvement in the resolution of chronic inflammatory lung and bowel diseases. LEI/SERPINB1 intrinsically possesses two enzymatic activities: an antiprotease activity dependent on its reactive site loop, which is analogous to the other proteins of the family and an endonuclease activity which is unveiled by the cleavage of the reactive site loop. The conformational change induced by this cleavage also unveils a bipartite nuclear localization signal allowing the protein to translocate to the nucleus. Recent data indicate that it has also a role in cell migration suggesting that it could be involved in diverse processes like wound healing and malignant metastases.
• Purity: >95%
• Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Pharmaceutical Information

• Applications: It is an intracellular protein and acts primarily to protect the cell from proteases released into the cytoplasm during stress. Its role in inflammation is clear due to its involvement in the resolution of chronic inflammatory lung and bowel diseases.
• Examples of Clinical Use: Protecting the cell from proteases released into the cytoplasm during stress.
• Affected organisms: Humans