Interleukin-8 (IL-8)

Specification

• Cat#: THP-0255
• Product Name: Interleukin-8 (IL-8)
• Description: Interleukin 8 (IL-8 or CXCL8) is a member of the CXC cytokine family and is produced by macrophages, epithelial, smooth muscle, and endothelial cells. IL-8 binds the G protein-coupled serpentine receptors CXCR1 and CXCR2. IL-8 recruits innate immune cells, induces phagocytosis, and stimulate angiogenesis. Recombinant human Interleukin 8 is a non-glycosylated protein monomer, containing 77 amino acids and having a molecular mass of 8.9 kDa.
• Sequences: AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
• Species: Human
• Molecular Weight: 8.9 kDa
• Source: E. coli
• Introduction: Interleukin 8 (IL-8) or CXCL8 is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors.
• Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/mL, which can be further diluted into other aqueous solutions.
• Application: It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived.

Related Reading

Background of IL-8

IL-8 protein, also known as interleukin-8, is a cytokine with inflammatory mediating effects. IL-8 was initially discovered in hamster ovarian cell lines and is widely distributed in various tissues in humans, such as lungs, liver, spleen, lymphatic tissue, kidneys, and intestines. IL-8 plays an important role as a chemokine in immune response and inflammatory processes.

The Function of IL-8

The main function of IL-8 is to induce the chemotaxis and activation of white blood cells during the inflammatory process, promote the migration of neutrophils and eosinophils, thereby increasing their concentration in the inflammatory site and accelerating the resolution of inflammation. In addition, IL-8 is also involved in inflammation and tumor generation, progression, and prognosis.

IL-8 Related Signaling Pathway

The signaling pathway of IL-8 mainly involves PI3K-AKT, MAPK, NF- κ B, these pathways ultimately lead to NF- κ The activation of B, transcription of IL-8 gene, translation of IL-8 protein, and a series of biological reactions ultimately lead to corresponding changes in the immune system, inflammation, and tumors.

IL-8 Related Diseases

IL-8 plays a role in many diseases, including infections, inflammation, autoimmune diseases, tumors, etc. In the process of infection and inflammation, IL-8 promotes the immune response of the body and helps eliminate pathogens by promoting chemotaxis and activating various white blood cells such as neutrophils. However, IL-8 may also play a role in transitional inflammation, leading to the occurrence of chronic inflammation and autoimmune diseases. In addition, the expression level of IL-8 in tumors is related to tumor progression and prognosis, and some studies suggest that inhibiting IL-8 expression may help suppress tumor growth and metastasis.

The Potential Application of IL-8 in Medicine

Due to its important role in inflammation and tumors, IL-8 has become an important drug target. At present, some drugs have been applied to inhibit IL-8 in order to improve the condition of inflammation and tumor patients. For example, some chemotherapeutic drugs (such as irinotecan) and some biological products (such as IL-8 antibody) have been proved to inhibit tumor growth and metastasis by inhibiting the expression or binding of IL-8. In addition, some studies have shown that regulating the expression and activity of IL-8 can improve the symptoms of certain autoimmune diseases and chronic inflammation.

List of Investigational Drugs Related To IL-8

Irinotecan: irinotecan is a chemotherapeutic drug widely used in patients with advanced tumors. It can inhibit tumor growth by inhibiting cell growth, and can also slow down tumor growth by inhibiting the expression of IL-8.

Elidel: Elidel is a drug used to treat allergic dermatitis, which can slow down allergic reactions and improve skin inflammation by inhibiting the expression of IL-8.

MK-7246: MK-7246 is a monoclonal antibody targeting IL-8 that is currently being studied. Its goal is to inhibit the binding of IL-8 to its receptor, thereby slowing down tumor growth and metastasis.