Fibroblast Growth Factor 2 (rFGF 2)

Specification

• Cat#: THP-0271
• Product Name: Fibroblast Growth Factor 2 (rFGF 2)
• Description: bFGF Rat Recombinant (FGF-2) produced in E Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.3 kDa.The bFGF2 is purified by proprietary chromatographic techniques.
• Sequences: MPALPEDGGG A FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
• Species: Rat
• Molecular Weight: 16.3 kDa
• Source: E. coli
• Introduction: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors. Recombinant fibroblast growth factor-2 (rFGF-2) improves perfusion in models of myocardial and hindlimb ischaemia.
• Purity: >97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to a specific activity of 5,000,000 IU/mg.
• Reconstitution: It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Application: Recombinant fibroblast growth factor-2 (rFGF-2) improves perfusion in models of myocardial and hindlimb ischaemia.