Botulinum toxin type B

Specification

• Cat#: THP-0028
• Product Name: Botulinum toxin type B
• Cas No: 93384-44-2
• Description: Neurotoxin produced by fermentation of clostridium botulinum type B. The protein exists in noncovalent association with hemagglutinin and nonhemagglutinin proteins as a neurotoxin complex. The neurotoxin complex is recovered from the fermentation process and purified through a series of precipitation and chromatography steps.
• Sequences: MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKEHLAVYKIQMCKSVKAPGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEEEKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCISKWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE
• Species: Clostridium botulinum
• Molecular Weight: 150804.0 Da
• Purity: >99% by SDS-Page and HPLC analysis
• Formula: Not Available
• Endotoxin: <0.01 EU per 1 μg of the peptide by the LAL method
• Application: For the treatment of patients with cervical dystonia to reduce the severity of abnormal head position and neck pain associated with cervical dystonia.

Pharmaceutical Information

• Pharmaceutical Application: Botulinum Toxin Type B
• Targets: Target 1. Vesicle-associated membrane protein 2; Target 2. Vesicle-associated membrane protein 1; Target 3. Synaptotagmin-2

Related Reading

Background of Botulinum Toxin Type B

Botulinum Toxin Type B (BoNT/B) is an intriguing biological molecule, with its roots tracing back to the discovery of the botulinum bacteria in the late 19th century by Dr. Émile van Ermengem. Since then, it has served as a significant player in the field of science and has transcended its stereotypic association with food poisoning to becoming an effective therapeutic tool in life transforming treatments.
Dr. Émile van Ermengem initially unearthed the botulinum bacteria in 1897 following an outbreak of food poisoning. He classified these gram-positive, anaerobic bacteria into different types named A-G, based on their production of seven distinctive neurotoxins. Botulinum toxin type B belongs to the botR gene locus, which plays a vital role in controlling the neurotoxin complexes' expression.
The protein structure of BoNT/B, similar to other toxin subtypes, comprises two chains, the heavy (~100 kDa) and light (~50 kDa) chain, connected by a disulphide bond. The heavy chain is instrumental in binding the toxin to the nerve cell surface, while the light chain acts as a protease, disrupting neurotransmitter release and inducing muscle paralysis.

Botulinum Toxin Type B Function and Related Signaling Pathways

Botulinum Toxin type B operates by inhibiting the release of acetylcholine, a crucial neurotransmitter involved in muscle contractions. It does this by cleaving the synaptosomal-associated protein 25 (SNAP-25) which prevents the fusion of the acetylcholine-containing vesicles with the cell membrane, thus prompting muscle relaxation.
Bont/B-related signaling pathways are majorly categorized into three: toxin endocytosis pathway; endosomal membrane transit pathway; and the translocation pathway, each possessing a specific function. The endocytosis pathway is responsible for the toxin's entry into the nerve cells; the endosomal membrane pathway addresses the toxin's translocation from the endosome into the cytosol; the translocation pathway delivers the toxin to its specific intracellular target.

Botulinum Toxin Type B Related Diseases & Its Role in Diseases

BoNT/B is notorious for causing botulism, a severe form of food poisoning marked by muscle paralysis. Nevertheless, the toxin’s potential for controlled use in treating medical conditions cannot be overemphasized. Researchers have uncovered its contributing role in conditions such as muscle spasticity disorders, cervical dystonia, or writer's cramp, gaining a clearer understanding of their pathophysiology.

The Application of Botulinum Toxin Type B in Medicine

Despite its toxic potential, the therapeutic applications of Botulinum toxin type B are numerous. Its ability to block neurotransmission seamlessly has found utility in managing various neurological conditions, including migraine, cerebral palsy, and hyperhidrosis. It has also demonstrated effectiveness in cosmetic applications, notable in treating conditions like facial wrinkles.

List of Drug Candidates Related to Botulinum Toxin Type B

Several botulinum toxin drugs are currently available on the market. Notably amongst them is Myobloc or Neurobloc, the first BoNT/B product approved by the Food and Drug Administration (FDA). Some other candidates under investigation include RimabotulinumtoxinB and Bont/B drugs conjugated with different targeting moieties aimed at reducing its systemic toxicity.
In conclusion, Botulinum toxin type B presents a fascinating dichotomy – its lethal potential balanced by its therapeutic possibilities. It is an epitome of the adage "the dose makes the poison". The future of medicine appears bright as scientists continue to explore and exploit this toxin's potential, hoping to unlock new therapeutic frontiers. The parasite that was once a foe is slowly but surely becoming a trusted friend.