Acidic Fibroblast Growth Factor (FGF-a)

Specification

• Cat#: THP-0232
• Product Name: Acidic Fibroblast Growth Factor (FGF-a)
• Description: Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of approximately 15.8kDa. The FGF acidic is purified by proprietary chromatographic techniques.
• Sequences: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
• Species: Human
• Molecular Weight: 15.8kDa
• Source: E. Coli
• Introduction: Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The hep-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
• Purity: >95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <0.5 ng/ml, corresponding to a specific activity of > 2,000,000IU/mg.
• Reconstitution: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 centigrade at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 centigrade, spin to remove precipitant.
• Application: This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.